Composite

Part:BBa_K2350011:Design

Designed by: CHIA-SUI CHIANG   Group: iGEM17_NYMU-Taipei   (2017-10-24)


Psb1c3-PrbcL-CrtZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used fusion PCR to fuse CrtZ and PrbcL to avoid restriction site in between which might influence expression efficiency.


Source

PrbcL is from Synechococcus elongatus PCC7942. CrtZ is from Pantoea ananatis.

References